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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been noticed in lots of tumors, which results in chromosome instability, and therefore may well lead to tumorigenesis. This protein was found to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant role in selling mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, happen to be described. Two extra splice variants had been noted but comprehensive nucleotide sequence data is not yet out there.
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Blocking peptides are peptides that bind specifically on the target antibody and block antibody binding. These peptide usually is made up of the epitope regarded from the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible success are...
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This guideline outlines further more possible optimization of cellular and immunoassay parameters to ensure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind towards the epitope around the target protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Goal Details Cyclin E belongs for the extremely conserved cyclin family members, whose users are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic party. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S changeover. This protein accumulates in the G1-S stage boundary and it is degraded as cells progress by S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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